Using Real Time PCR System, BGI has lots of achievements in Fusion Gene Diagnose of Leukemia, Gene Expression Analysis, Unknown Pathogen Identification and many other aspects. The platform of nucleic acid detection in BGI-GBI, Beijing, is mainly based on the real-time PCR technology to develop the kit of nucleic acid up to now, there have been forty kits of nucleic acid developed which including the SARS, novel H1N1 influenza by the platform.

 The real-time PCR system is based on the detection and quantitation of a fluorescent reporter. This signal increases in direct proportion to the amount of PCR product in a reaction. By recording the amount of fluorescence emission at each cycle, it is possible to monitor the PCR reaction during exponential phase where the first significant increase in the amount of PCR product correlates to the initial amount of target template.

AB 7300 Real-Time PCR System

The Applied Biosystems 7300 Real-Time PCR System gives you advanced, multicolor real-time PCR capabilities with exceptional budget sensitivity.

Four-color detection enables you to perform gene expression analysis, pathogen quantitation, SNP genotyping, and plus/minus assays with internal positive controls

Powerful, versatile software including plate set-up wizards and automated analysis tools make data processing simple and straightforward

Single excitation, four-emission filter optics and a charge-coupled device (CCD) camera with a sophisticated multicomponenting algorithm enable highly accurate, reproducible and reliable results

Peltier-based thermal cycling system allows you to use both standard 96-well plates and 0.2 mL tubes

Fluorescence For  ABI 7300 System:

• Reporter Dye: FAMTM, VICTM, JOETM, SYBR GREEN

• Quencher Dye: TAMRATM, NEDTM

• Passive Reference: ROCTM

          7300 Real-Time PCR System                     PCR stent

AB Stepone real-time PCR system

The StepOne and StepOnePlus systems are using the latest graphical user interface and design friendly. Because of the Wizard function to facilitate, the people who have even never used real-time PCR instrument amplification experiments can convenient set up and analysis of experiments. To simplify the operation, system also is equipped with a touch screen display. You just need to click on the touch screen a few. Then it can quickly launch a real-time PCR reaction.

The StepOne and StepOnePlus Systems also have another feature. That is simplifying the software design. The software designer comprehensively consider the demand of user. It has all the needs of real-time PCR experiments operating function. For example, Absolute quantification, relative quantification (relative standard curve and comparative CT method), SNP genotyping, presence/absence assays.In order to ensure the reliability of the experiment, software can also automatically calculate the amount of reagents and standards of the dilution curve, and give you the pipette program. At last, it can analysis the final results for you.

The software is so comprehensive. It is simple and flexible, and it can the most significantly reduce the error probability. That makes me get the reliable and high-quality results. It can be suitable for different users’ experience. There is a wizard function in the StepOne system. The wizard function can specially designed specific experiments for different people. And it can guide the news to design the experiments. And it also can set personalized design parameters for the experimental skilled staff. For example, Thermal cycling process, Nucleic acid template types.

     Stepone real-time PCR system              Stepone Software V2