The SOLiD™3 System is a highly accurate, massively parallel next-generation sequencing platform that supports a wide range of applications. This technique is based on the polymerization reaction with oligo nucleotides labeled by four fluorescence color. It can polymerize and amplify single copy DNA and sequence enormous DNA fragments. With unparalleled throughput and greater than 99.94% basecalling accuracy, the SOLiD™3 System enables you to make research in small RNA analysis, whole transcriptome analysis Chromatin immunoprecipitation sequencing( ChIP-Seq), methylation analysis.

Workflow:

1. Library Preparation

Two type libraries:

• Fragment  library

• Mate-paired  library

 2.  Emulsion PCR/Bead Enrichment

 • Emulsion PCR

• Amplification of DNA fragments on magnet beads

3.  Bead Deposition

• Random distribution of magnetic DNA beads

• Increasing densities of beads per slide

4.  Synthesis-based-sequencing

• Oligo nucleotides labeled by four fluorescence color

• SBS (synthesis-based-sequencing) on SOLiD™3 analyzer

5. Primer Reset

• Five rounds of primer reset are completed for each sequence tag

Features:

• High-throughput: One run of SOLiD™ 3 System can generate data up to 20G, 400M Tags;

• High accuracy: Double reads of each nucleotide, accurate reading of each nucleotide;

• High stability: SBS is good for polynucleotide sequences;

• The flexibility of two independent flow cells and barcodes allow you to conduct multiple experiments in a single run.