BGI has the largest number of Sanger sequencing service division in China, providing high quality results at a high speed; With 18 Applied Biosystems 3730XL DNA analyzer located worldwide, our through output can be 500 thousand reactions per month, which is increasing with the expanding steps of BGI. The analyzer can provide high quality read lengths of up to 1100 QV20 bases, and the reliability of every single base it produces is extremely high. As the application of the analyzer is extensive and flexible, fragment analysis services such as SNP, SSR, STR can also provided besides normal DNA analysis.
We have production centers of excellence and customer service in all major regions of the world supporting our global customers’ needs.
China：beijing, Qingdao, wuhan, shanghai, Guangzhou, Shenzhen.
International：Kongeriget Danmark , Czeh(Arrangement).
Small genome sequencing shotgun library construction & sequencing.
Figure 1, ABI 3730XL DNA Analyzer
Figure 2, Sequence principle of ABI3730XL
Information of DNA Analyzer
1. Dual beam laser excitation both sides, the rear grating devices and detection of ultra-thin CCD imaging system
2. Totally automated during sequencing, 24 hours nonstop;
3. 96 samples per run , daily output more than 1152 samples;
4. Using latest POP7 fluid gel, dynamic capillary inner wall coating, produce high quality read lengths of up to 1100 QV20 bases;
5. Electrophoresis temperature range 18-70℃, high temperature help to reduce the impact of secondary structure;
6. New base identification and quality score software to improve sequencing accuracy;
7. High sensitivity of detection of DNA in the concentration range. Ultra-low cost sequencing platforms.
Services & Products
Molecular Biology Services
v Single-molecule sequencing;
v Shotgun Library Construction & Sequencing;
v Fosmid Library Construction/BAC & Fosmid terminal sequencing;
v Full-length cDNA Library Construction/Normalized cDNA Libra ry Construction /Yeast Two-Hybrid Library Construction;
v PCR Re-sequencing/SNP Genotyping /SSR detection/Walking sequencing;
v TA cloning/Vector Construction;
v Species identification(Bacteria or Fungi)/DNA Barcoding;
v Mitochondrial/chloroplast/plasmid/phage Sequencing;
v DNA/RNA virus Sequencing;
v DNA/RNA extraction from the source or tissue type;
v Fluorescent dye-terminator sequencing performed on 3730XL sequencers.
v BGI has established a quality management system and has passed ISO 9001 system certification of quality management;
v BGI has bioinformatic teams could provide customized bioinformatics analysis services to solve any problems from customers.
Workflow of the shotgun Library Construction and Sequencing
v Shotgun library construction has the following applications, virus, phage, plasmid, chloroplast, mitochondrial, BAC/Fosmid clone sequencing;
v Sequences reliable.It can remove the influence of secondary structure and sequence the genome sequence of unknown or little known, then high throughput sequencing technology can’t settle.
Full-length cDNA Library Construction and EST Sequences
v Utilizing Clontech’s SMART technology BGI’s provides our clients with high-quality, truly full-length libraries;
v Normaliztion decreases the prevalence of high abundance transcripts and equalizes transcript concentrations, increasing the efficiency of large-scale sequencing and rare gene screening;
v Using different vector with Yeast Two-Hybrid Library Construction.
Flow chart of the cDNA Library Construction
v New gene cloning;
v Analysis of gene expression profiles;
v Revealing SSR markers from an EST database;
v Exploring key genes related to disease prevention and development function;
v Performing transgenic function research to change a creature’s characters;
v Protection of endangered treasure biological resources, establishment of gene library of the creature;
v An EST can be used as probes to construct the molecular marked linkage map.
v Accurate sequencing results , the “gold standard” for re-sequencing;
v Widely used in SNP detection, virus re-sequencing and other aspects;
v Not only can detect known mutation gene locus, but also the unknown locus;
v Flexible experimental design and can be designed for small samples;
The Flowchart of PCR Re-sequencing
v Monogenic inherited diseases: pathogenic genetic research and genetic diagnosis;
v Complex diseases: diseases-associated genes research and drug susceptibility analysis;
v Correlation analysis between mutations, deletions, insertions, and other changes in the nucleic acid level and phenotype .
Fosmid Library Production
v The Insert DNA is in the 33-45k size range, able to accommodate most of the genes for genetic screening;
v Fosmid vectors are maintained as single copy in the host bacteria to ensure stability and not easy to recombinate;
v The clones can be induced to high copy number and will be conducive to downstream applications.
The Flowchart of Fosmid Library production
v Whole genome physical map construction;
v Gene function ,gene expression and regulation research;
v Positional cloning of genes associated with important traits ;
v Gene structure and function analysis.